Thus, well-characterized constitutive or inducible promoters with strong transcriptional activity are generally used to achieve overproduction of recombinant proteins.
The well-characterized and strong constitutive ., 2010).
The recombinant hepatitis B vaccine produced in the nonconventional yeast -produced hepatitis B virus S antigen (Hbs Ag) was found to be assembled into yeast-derived lipid membranes.
Previous studies have indicated that this lipoprotein particle structure is essential for the antigenicity of the HBs Ag (Rutgers was approved by the FDA in 2009, and several others are undergoing evaluation in clinical trials (Walsh 2010a).
Inducible promoter systems offer the advantage of controlling gene expression levels in response to the presence of specific inducer or repressor molecules (Shen promoters.
Although such native promoter systems provide well-defined, predictable expression profiles, a disadvantage of these promoters is that the inducer molecule, galactose, is consumed as a carbon source by yeast cells, which further complicates the control of gene expression.
The traditional baker's yeast is one of the best-characterized eukaryotes and most widely used host systems for biopharmaceutical production since the early days of genetic engineering and recombinant protein production (Martinez ., 2013), facilitating functional genomics analysis based on various ‘omics’ techniques.
Systems biology-based integrated functional genomics could provide a deeper, more holistic understanding of yeast biology, which can help identify potential limitations in protein production on a global scale.
Yeasts are capable of robust growth on simple media, readily accommodate genetic modifications, and incorporate typical eukaryotic post-translational modifications.
have gained increasing attention as alternative hosts for the industrial production of recombinant proteins.